Herramientas de Accesibilidad
Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment. The work sought to evaluate the antimicrobial activity of the three substances (Cymbopogon citratus essential oil and the citral and myrcene components) against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. Through Scanning electron microscopy, the typical morphology of S. mutans was observed and, a characteristic formation of polysaccharides, with more structured arrangement at 48 h. The bacterial viability effect against S. mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1 % as vehicle control (positive control). The three substances evaluated had effects on the bacterial viability of S. mutans with mortality between 74 and 96 %, without significant difference among them (P > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24 h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects.
Revista de Biologia Tropical
Universidad de Santander UDES. Vigilada Mineducación.
Resolución otorgada por el Ministerio de Educación Nacional: No. 6216 del 22 de diciembre de 2005 / Personería Jurídica 810 de 12/03/96.
Institución sujeta a inspección y vigilancia por el Ministerio de Educación Nacional. Resolución 12220 de 2016.
Notificaciones administrativas y judiciales:
Copyright © 2021 - Todos los derechos reservados